Instruments at ALEC

FluoroMax-4 Spectrofluorometer

Steady-state fluorescence spectroscopy investigates the long-term average fluorescence of a sample when irradiated with UV, visible, or near-IR light. The FluoroMax-4 is a compact spectrofluorometer with high sensitivity. The sample chamber allows a wide variety of accessories for special samples and allows experimentation with different sample schemes. FluoroMax-4 is controlled by PC driven FluorEssence™ software. On start-up, the system automatically calibrates and presents itself for new experiments, or stored routines called from memory. Publication-ready plots and data-analysis are based on Origin®.

Specifications include:

• Excitation: 200–950 nm, optimized in the UV
• Emission: 200–950 nm, optimized in the visible
• Bandpass: 0–30 nm, continuously adjustable from computer
• Wavelength Accuracy: ± 0.5 nm
• Scan Speed: 80 nm/s
• Integration Time: 1 ms to 160 s
• Emission Detector: Photomultiplier, range 200–850 nm
• Reference Detector: Photodiode selected for stability
• Signal-to-Noise Ratio: 3000:1 (steady-state mode)